Coding

Part:BBa_K5222009

Designed by: Maria Avtodeeva   Group: iGEM24_Sorbonne-U-Paris   (2024-10-02)


CDS of HsOMT adapted for B3-B5 position of MoClo

In the article Biological Valorization of Poly(ethylene terephthalate) Monomers for Upcycling Waste PET, the authors identified a Homo sapiens O-methyltransferase (HsOMT) enzyme as the most efficient protein for converting protocatechuic acid (PCA) into vanillic acid (VA). Ana Rita Brochado et al. also reported that HsOMT plays a crucial role in vanillin production and that overexpressing this enzyme in a strain with model-guided modifications can significantly increase the final product yield.

Essentially, this enzyme catalyses the methylation reaction, where a methyl group is added to the hydroxyl group on the PCA molecule, converting it into vanillic acid. Here we created a CDS of HsOMT adapted for B3-B5 position of MoClo


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 52
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 52
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 52
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 52
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None